①硝酸还原酶易失活,粗酶液制备过程需在冰上迅速完成;
②空白组为各试剂组分质量的检测孔,正常情况下变化不超过0.05;
③准确在规定时间点完成吸光值的测定,以确保实验结果的准确性和重复性;
④试剂三有效期较短,为便于试验安排附赠1瓶作为备用,每瓶均可满足100个样本的测定;
⑤若A测定大于1.2或者∆A大于0.5时,建议将粗酶液适当稀释后再进行测定;若∆A测定小于0.01,可适当延长酶促反应时间后再进行测定,计算时相应修改;
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