Laccase Activity Assay Kit
漆酶活性检测试剂盒
货号:AKAO018M
规格: 110T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Laccase Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:5 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
漆酶能够催化ABTS生成ABTS自由基,产物在420 nm处具有特征吸收峰,通过吸光值变化速率即可表征漆酶的活性。
  • 检测方法: ABTS法
  • 检测波长: 420 nm
  • 信号响应: 递增型
Notices

①若A2测定大于1.0,建议将粗酶液使用提取液适当稀释后再进行测定,计算时相应修改;

②空白管为检测各试剂组分质量的检测孔,正常情况下,OD值变化不超过0.05;

③准确在10 s和190 s完成吸光值测定,以确保实验结果的准确性和重复性;若使用96孔板测定应使用多道移液器且分批测定,以确保组间反应时间一致;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Zhang Y, Huang S, Cheng Y, et al. Enhanced lignocellulose degradation and composts fertility of Flammulina filiformis residue and eco-friendly insect (Protaetia brevitarsis) composting by microbial agents-loaded biochar[J]. Chemical Engineering Journal, 2025: 164126. (IF 13.3)

[2] Wang S, Long H, Hu X, et al. The co-inoculation of Trichoderma viridis and Bacillus subtilis improved the aerobic composting efficiency and degradation of lignocellulose[J]. Bioresource Technology, 2024, 394: 130285.(IF 11.4)

[3] Lü X P, Lü Z L, Zhang Y M, et al. Lignin synthesis plays an essential role in the adaptation of Haloxylon ammodendron to adverse environments[J]. International Journal of Biological Macromolecules, 2025: 142321. (IF 7.7)

[4] Zhang H, He Z, Zhang X, et al. A Highly Sensitive Caffeic Acid Fluorescent Probe for Detecting Laccase in Grape Juice and Mushrooms[J]. Analyst, 2025. (IF 3.6)

[5] Hu M, Yuan L, Cai Z, et al. Ammonia fiber expansion combined with white rot fungi to treat lignocellulose for cultivation of mushrooms[J]. ACS omega, 2021, 6(47): 31689-31698.(IF 3.512)

[6] Song J Y, Wu H X, Li J C, et al. Taxonomy and evolution history of two new litter-decomposing Ciliochorella (Amphisphaeriales, Sporocadaceae)[J]. MycoKeys, 2023, 100: 95.(IF 3.3)

[7] Li Y, Zhang R, Sun L, et al. Resistance of Populus davidiana× P. bolleana overexpressing cinnamoyl-CoA reductase gene to Lymantria dispar larvae[J]. Transgenic Research, 2025, 34(1): 10. (IF 2.7)

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