

①若A2测定大于1.0,建议将粗酶液使用提取液适当稀释后再进行测定,计算时相应修改;
②空白管为检测各试剂组分质量的检测孔,正常情况下,OD值变化不超过0.05;
③准确在10 s和190 s完成吸光值测定,以确保实验结果的准确性和重复性;若使用96孔板测定应使用多道移液器且分批测定,以确保组间反应时间一致;
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