

①若A1测定大于1.2或ΔA大于0.5,建议将粗酶液使用提取液适当稀释后再进行测定;若ΔA小于0.02,建议适当延长酶促反应时间(37℃准确反应时间,可延长至30-60 min)或适当增加样本量后再进行测定,计算时相应修改;
②因提取液中含有干扰蛋白检测组分,提取后粗酶液不能用于蛋白含量的测定,应另取组织使用PBS或生理盐水单独提取后再进行测定;
③准确在规定时间点完成吸光值的测定,以保证实验结果准确性和重复性;
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