Lipase (LPS) Activity Assay Kit
脂肪酶活性检测试剂盒
货号:AKFA006C
规格: 70T/50S
检测设备:可见分光光度计
可检测样本数:50 Samples
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Product Information
Lipase (LPS) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:6 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    无水乙醇(C2H6O,MW = 46.07,CAS: 64-17-5);
    甲苯(C7H8,MW = 92.14,CAS: 108-88-3)
Detection Principle
脂肪酶催化油酯水解生成游离脂肪酸,利用铜皂法测定脂肪酸生成速率:脂肪酸与显色剂中铜离子反应生成铜皂蓝色络合物,产物在710 nm处具有特征吸收峰,通过吸光值变化即可表征脂肪酶的活性。
  • 检测方法: 铜皂法
  • 检测波长: 710 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Oleic Acid
  • 参考标准: y=0.0252x+0.0601 (R2=0.9994)
  • 标准线性范围: 1.875-60 µmol/mL
  • 检测限: To Be Updated
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①实验过程中须远离火源,甲苯具有毒性,建议在通风橱中操作并做好防护措施;

②若测定吸光值超出标准吸光值线性范围:高于最高值建议将粗酶液使用试剂一适当稀释后再进行测定,低于最低值建议适当增加样本量后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Yu S, Jing Y, Fan Y, et al. Ultrahigh efficient emulsification with drag-reducing liquid gating interfacial behavior[J]. Proceedings of the National Academy of Sciences, 2022, 119(29): e2206462119.(IF 12.779)

[2] Yang Y, Wu Y, Huang J, et al. A novel type III effector RipBU from Ralstonia solanacearum suppresses plant immunity and promotes peanut susceptibility[J]. International Journal of Biological Macromolecules, 2024: 138189. (IF 7.7)

[3] Xu L, Zhao J, Xu D, et al. Application of transcriptomic analysis to unveil the toxicity mechanisms of fall armyworm response after exposure to sublethal chlorantraniliprole[J]. Ecotoxicology and Environmental Safety, 2022, 230: 113145.(IF 6.291)

[4] Chen X L, Wu L J, Miao L L, et al. Chronic polystyrene microplastics exposure-induced changes in thick-shell mussel (Mytilus coruscus) metaorganism: A holistic perspective[J]. Ecotoxicology and Environmental Safety, 2024, 284: 116961. (IF 6.2)

[5] Liu X, Xie H, Xu Y, et al. Two halogenated flame retardants and cadmium in the soil-rice system: sorption, root uptake, and translocation[J]. Environmental Science and Pollution Research, 2023, 30(43): 97688-97699.(IF 5.8)

[6] Yao Y, Li M, Liu Q, et al. The Virulence of Metarhizium rileyi to Locusta migratoria Is Determined by the Ability of the Fungus to Respond to Carbon and Nitrogen Sources[J]. International Journal of Molecular Sciences, 2025, 26(9): 4156. (IF 4.9)

[7] Xu L, Liu B, Xiao L, et al. Comparative toxicity mechanisms of sulfoxaflor and lambda-cyhalothrin against Apolygus lucorum from enzymatic and transcriptomic perspectives: Efficient application of insecticides[J]. Pesticide Biochemistry and Physiology, 2025: 106306. (IF 4.2)

[8] Wang F, Jiang X, Zhao Z, et al. Neurokinin B is a potential target for treating disruption of intestinal mucosal barrier in acute mechanical intestinal obstruction[J]. Peptides, 2025: 171419. (IF 2.9)

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