Ascorbate Peroxidase (APX) Activity Assay Kit
抗坏血酸过氧化物酶活性检测试剂盒
货号:AKVI006M
规格: 110T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Ascorbate Peroxidase (APX) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔UV板
  • 预计测定时间:5 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
抗坏血酸过氧化物酶可催化H2O2氧化抗坏血酸生成单脱氢抗坏血酸,通过测定抗坏血酸的氧化速率即可表征抗坏血酸过氧化物酶的活性。
  • 检测方法: AsA比色法
  • 检测波长: 290 nm
  • 信号响应: 递减型
Notices
①准确在10 s和130 s处完成读数,以保证实验结果的准确性;若使用96孔UV板进行测定,应使用多道移液器分批进行测定,以确保组间反应时间一致;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Li C, Li J, Du X, et al. Chloroplast thylakoidal ascorbate peroxidase, PtotAPX, has enhanced resistance to oxidative stress in Populus tomentosa[J]. International Journal of Molecular Sciences, 2022, 23(6): 3340.(IF 6.208)

[2] Yao X, Zhou M, Ruan J, et al. Physiological and Biochemical Regulation Mechanism of Exogenous Hydrogen Peroxide in Alleviating NaCl Stress Toxicity in Tartary Buckwheat (Fagopyrum tataricum (L.) Gaertn)[J]. International Journal of Molecular Sciences, 2022, 23(18): 10698.(IF 6.208)

[3] Li J, Cai B, Chang S, et al. Mechanisms associated with the synergistic induction of resistance to tobacco black shank in tobacco by arbuscular mycorrhizal fungi and β-aminobutyric acid[J]. Frontiers in Plant Science, 2023, 14.(IF 5.6)

[4] Shen L, Zhou Y, Yang X. Genome-wide identification of ascorbate peroxidase (APX) gene family and the function of SmAPX2 under high temperature stress in eggplant[J]. Scientia Horticulturae, 2024, 326: 112744.(IF 4.3)

[5] Xu J, Kang Z, Zhu K, et al. RBOH1-dependent H2O2 mediates spermine-induced antioxidant enzyme system to enhance tomato seedling tolerance to salinity–alkalinity stress[J]. Plant Physiology and Biochemistry, 2021, 164: 237-246.(IF 4.27)

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