Granule-Bound (GBSS) Starch Synthase Activity Assay Kit
结合态淀粉合成酶活性检测试剂盒
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Product Information
Granule-Bound (GBSS) Starch Synthase Activity Assay Kit
-20℃ Dry Ice Transportation
- 检测样本量：96 Samples
- 主要检测设备及配套：酶标仪/96孔UV板
- 预计测定时间：4 h (100 Samples)
- 试剂储存条件：按照标签要求储存
-
需自备试剂:无
Detection Principle
结合态淀粉合成酶催化ADPG与淀粉引物（葡聚糖）反应，将葡萄糖分子转移到淀粉引物上并生成ADP，再通过丙酮酸激酶、己糖激酶和6-磷酸葡萄糖脱氢酶依次催化NADP+还原为NADPH，NADPH在340 nm处具有特征吸收峰，通过吸光值的变化即可表征结合态淀粉合成酶活性。
- 检测方法： NADPH速率法
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检测波长： 340 nm
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信号响应： 递增型
Notices
注： 为保证结果准确且避免试剂损失，测定前请仔细阅读说明书（以实际收到说明书为准），确认试剂储存和准备是否充分，操作步骤是否清楚，且务必取2-3个预期差异交的样本进行预测定，过程中问题请您及时与工作人员联系。
Product Citation
[1] Wang L, Mao Y, Zhou S, et al. Understanding the amylose biosynthesis and regulation mechanisms in Tartary buckwheat by the endosperm transcriptome[J]. International Journal of Biological Macromolecules, 2024: 135275. (IF 7.7)
[2] Ma H, Jia Y, Wang W, et al. Effects of Low-Temperature Stress During the Grain-Filling Stage on Carbon–Nitrogen Metabolism and Grain Yield Formation in Rice[J]. Agronomy, 2025, 15(2): 417. (IF 3.3)
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The Dilution Calculator Equation
Concentration (start)xVolume (start)= Concentration (final)× Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
This equation is commonly abbreviated as: C1V1 = C2V2
Sample(S):可测定的样品数量；
★根据此数量计算需要的试剂盒个数。
Size
100T/96S
Test(T):总反应次数；
★每个试剂盒均预留了至少5个（分光光度法）和10个（微板法）样本预实验的试剂用量；
★若需要制作标准曲线则至少能够满足2次制作标准曲线的试剂用量（15个）。
