Bisphosphate Carboxylase/Oxygenase (Rubisco) Activity Assay Kit
二磷酸核酮糖羧化酶/加氧酶活性检测试剂盒
货号:AKPL001U-2
规格: 50T/48S
检测设备:紫外分光光度计
可检测样本数:48 Samples
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Product Information
Bisphosphate Carboxylase/Oxygenase (Rubisco) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:48 Samples
  • 主要检测设备及配套:紫外分光光度计/1 mL石英比色皿(d=10 mm)
  • 预计测定时间:5 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
Rubisco可催化核酮糖-1,5-二磷酸(RuBP)和CO2结合生成3-磷酸甘油酸,3-磷酸甘油酸可进一步在3-磷酸甘油酸激酶和甘油醛-3-磷酸脱氢酶的催化作用下生成甘油醛-3-磷酸,反应过程中伴随着NADH氧化生成NAD+,NADH在340 nm处有特征吸收峰,通过测定340 nm处吸光度的下降速率即可表征Rubisco活性。
  • 检测方法: NADH速率法
  • 检测波长: 340 nm
  • 信号响应: 递减型
Notices
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Zhao H, Abulaizi A, Wang C, et al. Overexpression of CgbHLH001, a positive regulator to adversity, enhances the photosynthetic capacity of maize seedlings under drought stress[J]. Agronomy, 2022, 12(5): 1149.(IF 3.949)

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