Total RNA Extraction Kit
总RNA提取试剂盒
货号:AKNA001-1
规格: 50T
检测设备:-
可检测样本数:50 Samples
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Product Information
Total RNA Extraction Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:匀浆器、冰水浴、低温离心机、离心管、可调式移液器
  • 预计测定时间:-
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    无水乙醇(C2H6O,MW=46.07,CAS: 64-17-5);
    液氮
Detection Principle
本产品适用于各种动植物组织、细胞、细菌、酵母和丝状真菌中提取总RNA,提取的总RNA分子完整、纯度高,适用于Northern Blot、Dot Blot、RT-PCR、Primer Extension、PolyA筛选、S1核酸酶作图、RNase保护测定、体外翻译、构建cDNA文库等分子生物学试验。
  • 检测方法: -
  • 检测波长: -
  • 信号响应: -
Notices

①所有相关器皿耗材都应为RNase-free产品,操作过程要小心,带口罩、手套避免环境中RNA酶污染样品;

②RNA在水溶液中OD值可能在1.5-1.9之间,但这并不表示RNA不纯,需电泳检测;

③组织若不经过处理,RNA易被降解,新鲜组织应及时放入液氮中速冻并立即储存于-70℃,冻存的组织不能反复冻融以避免RNA的降解,样品也可以在匀浆后加入裂解液,储存于-70℃;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Huang L, Jia X, Zhao Y, et al. Effects of elevated CO2 on phenolics in black locust seedlings inoculated with arbuscular mycorrhizal fungi under cadmium pollution by 13C isotopic tracer technique[J]. Plant and Soil, 2023: 1-20.(IF 4.9)

[2] Zhao S Y, Zhao H H, Wang B H, et al. Rhein alleviates advanced glycation end products (AGEs)-induced inflammatory injury of diabetic cardiomyopathy in vitro and in vivo models[J]. Journal of Natural Medicines, 2023, 77(4): 898-915.(IF 3.3)

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