Glutamic Oxalacetic Transaminase (GOT) Activity Assay Kit
谷草转氨酶活性检测试剂盒
货号:AKAM019M
规格: 120T/50S
检测设备:酶标仪
可检测样本数:50 Samples
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Product Information
Glutamic Oxalacetic Transaminase (GOT) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
谷草转氨酶催化α-酮戊二酸和天门冬氨酸发生转氨基反应,生成谷氨酸和草酰乙酸,草酰乙酸进一步自行脱羧生成丙酮酸,丙酮酸能够与2,4-二硝基苯肼反应生成2,4-二硝基苯腙,在碱性条件下呈棕红色,产物在505 nm处具有特征吸收峰,通过吸光值的变化即可表征谷草转氨酶的活性。
  • 检测方法: 2,4-二硝基苯肼法
  • 检测波长: 505 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Sodium Pyruvate
  • 参考标准: y=0.3978x+0.0098 (R2=0.999)
  • 标准线性范围: 0.05-1.6 μmol/mL
  • 检测限: -
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
①若测定吸光值超出标准线性吸光范围:高于最高值建议将粗酶液适当稀释后再进行测定;低于最低值建议适当增加样本量后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

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