Phenylalanine Ammonia-Lyase (PAL) Activity Assay Kit
苯丙氨酸解氨酶活性检测试剂盒
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Product Information
Phenylalanine Ammonia-Lyase (PAL) Activity Assay Kit
4℃ Wet Ice Transportation
- 检测样本量：100 Samples
- 主要检测设备及配套：酶标仪/96孔UV板
- 预计测定时间：4 h (100 Samples)
- 试剂储存条件：按照标签要求储存
-
需自备试剂:无
Detection Principle
苯丙氨酸解氨酶可催化L-苯丙氨酸裂解为反式肉桂酸和氨，反式肉桂酸在290 nm处具有特征吸收峰，通过吸光值的变化速率即可表征苯丙氨酸解氨酶的活性。
- 检测方法： 反式肉桂酸比色法
-
检测波长： 290 nm
-
信号响应： 递增型
Notices
注： 为保证结果准确且避免试剂损失，测定前请仔细阅读说明书（以实际收到说明书为准），确认试剂储存和准备是否充分，操作步骤是否清楚，且务必取2-3个预期差异交的样本进行预测定，过程中问题请您及时与工作人员联系。
Product Citation
[1] Fan L, Shi G, Yang J, et al. A Protective Role of Phenylalanine Ammonia-Lyase from Astragalus membranaceus against Saline-Alkali Stress[J]. International Journal of Molecular Sciences, 2022, 23(24): 15686.(IF 6.208)
[2] Li X, Xiu D, Huang J, et al. Nutshell Physicochemical Characteristics of Different Hazel Cultivars and Their Defensive Activity toward Curculio nucum (Coleoptera: Curculionidae)[J]. Forests, 2023, 14(2): 319.(IF 2.9)
[3] Hou S, Liu Z, Li Y, et al. Exogenous salicylic acid enhanced resistance of Foxtail Millet (Setaria italica) to Sclerospora graminicola[J]. Plant Growth Regulation, 2023, 99(1): 35-44.(IF 3.242)
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The Dilution Calculator Equation
Concentration (start)xVolume (start)= Concentration (final)× Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
This equation is commonly abbreviated as: C1V1 = C2V2
Sample(S):可测定的样品数量；
★根据此数量计算需要的试剂盒个数。
Size
110T/100S
Test(T):总反应次数；
★每个试剂盒均预留了至少5个（分光光度法）和10个（微板法）样本预实验的试剂用量；
★若需要制作标准曲线则至少能够满足2次制作标准曲线的试剂用量（15个）。
