Proline (Pro) Content Assay Kit
脯氨酸含量检测试剂盒
货号:AKAM003M
规格: 120T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Proline (Pro) Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:4 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    冰乙酸(C2H4O2,MW=60.05,CAS:64-19-7);
    甲苯(C7H8,MW=92.14,CAS:108-88-3);
Detection Principle
脯氨酸经磺基水杨酸提取后,与酸性茚三酮反应生成红色物质,产物经甲苯萃取,通过测定520 nm处吸光值变化即可定量检测脯氨酸的含量。
  • 检测方法: 酸性茚三酮法
  • 检测波长: 520 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Proline
  • 参考标准: y =0.0432x+0.0273 (R2=0.9997)
  • 标准线性范围: 1.25-30 μg/mL
  • 检测限: 1 μg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①提取液中含有蛋白沉淀剂,若采用蛋白浓度计算结果,需单独使用PBS提取后再测定蛋白浓度;

②若A测定或∆A测定超出标准吸光值线性范围:高于最高值建议将待测样本使用提取液适当稀释后再进行测定,低于最低值建议适当调整样本量和比例重新提取后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

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