Sucrose Synthase (SS) Activity Assay Kit
蔗糖合成酶活性检测试剂盒
货号:AKSU021C
规格: 70T/25S
检测设备:可见分光光度计
可检测样本数:25 Samples
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Product Information
Sucrose Synthase (SS) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:25 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:3 h (25 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
蔗糖合成酶催化游离果糖与葡萄糖供体UDPG反应生成蔗糖,蔗糖与间苯二酚反应生成有色物质,产物在480 nm处具有特征吸收峰,通过吸光值变化即可表征蔗糖合成酶的活性。
  • 检测方法: 间苯二酚法
  • 检测波长: 480 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Sucrose
  • 参考标准: y=0.4428x+0.0075 (R2=0.9994)
  • 标准线性范围: 0.125-3 mg/mL
  • 检测限: To Be Updated
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①若测定吸光值超出标准线性吸光值范围:高于最高值建议将粗酶液适当稀释后再进行测定;低于最低值建议适当增加样本量重新制备粗酶液后再进行测定,计算时相应修改;

②显色完成后应在30 min内完成测定;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Yu S, Jing Y, Fan Y, et al. Ultrahigh efficient emulsification with drag-reducing liquid gating interfacial behavior[J]. Proceedings of the National Academy of Sciences, 2022, 119(29): e2206462119.(IF 12.779)

[2] Yue Y, Sun S, Li J, et al. GmFULa improves soybean yield by enhancing carbon assimilation without altering flowering time or maturity[J]. Plant Cell Reports, 2021, 40: 1875-1888.(IF 4.57)

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