Soil Alkaline Phosphatase (S-AKP/ALP) Activity Assay Kit
土壤碱性磷酸酶活性检测试剂盒
货号:AKEN027C
规格: 70T/50S
检测设备:可见分光光度计
可检测样本数:50 Samples
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Product Information
Soil Alkaline Phosphatase (S-AKP/ALP) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:25 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    无水乙醇(C2H6O,MW=46.07,CAS: 64-17-5);
    甲苯(C7H8,MW=96.14,CAS:108-88-3)
Detection Principle
土壤碱性磷酸酶(S-AKP/ALP)能够在碱性环境中催化磷酸苯二钠水解生成苯酚和磷酸氢二钠,通过测定酚的生成量即可表征土壤碱性磷酸酶的活性。
  • 检测方法: 磷酸苯二钠法
  • 检测波长: 660 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Phenol
  • 参考标准: y=0.7648x+0.0215(R2=0.9992)
  • 标准线性范围: 0.1-1.0 μmol/mL
  • 检测限: To Be Updated
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
①若测定吸光值超出标准吸光值线性范围:高于最高值建议将待测样本适当稀释后再进行测定,低于最低值建议适当增加样本后再进行测定,计算时相应修改即可;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Li X, Fan S, Zhang Y, et al. Performance and microbial metabolic mechanism of imidacloprid removal in a microbial electrolysis cell-integrated adsorption biological coupling system[J]. Bioresource Technology, 2023, 386: 129513.(IF 11.4)

[2] Huang B, Chen Y, Pei Z, et al. Application of microbial organic fertilizers promotes the utilization of nutrients and restoration of microbial community structure and function in rhizosphere soils after dazomet fumigation[J]. Frontiers in Microbiology, 2023, 13: 1122611.(IF 5.2)

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