①A1应控制在0.1-1(96孔板0.05-0.5)范围内,可提高检测灵敏度:若A1大于1(96孔板大于0.5),建议适当减少反应体系中待测样本的添加量,保持总体积200 μL不变(如10 μL待测样本和190 μL试剂一/试剂二);若A1小于0.1(96孔板小于0.05),建议适当增加反应体系中待测样本的添加量,保持总体积200 μL不变(如100 μL待测样本和100 μL试剂一/试剂二),计算时相应修改V样的体积;
②提取液会使蛋白变性,若使用蛋白浓度计算需用PBS单独提取后进行测定;[1] Hao Y, Wang Q, Abbas M, et al. PagMYB128 regulates anthocyanin biosynthesis by directly activating PagMYB116 in Populus alba× Populus glandulosa[J]. Industrial Crops and Products, 2024, 218: 119046. (IF 5.6)
[2] Zeng Z, Zou Y, Lin F C, et al. Roles of CcDFR and CcOMT9 in the cyanidin biosynthesis and development of Cordyceps cicadae[J]. Frontiers in Microbiology, 2024, 15: 1353710. (IF 5.2)
[3] Cheng H, Chang S, Shi X, et al. Molecular Mechanisms of the Effects of Sodium Selenite on the Growth, Nutritional Quality, and Species of Organic Selenium in Dandelions[J]. Horticulturae, 2024, 10(3): 209. (IF 3.1)