Plant Anthocyanin Content Assay Kit
植物花色苷含量检测试剂盒
货号:AKPL021M
规格: 110T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Plant Anthocyanin Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96 孔板
  • 预计测定时间:3 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
采用pH示差法测定花色苷含量:当pH=1.0时花色苷在530 nm处具有特征吸收峰,当pH=4.5时,花色苷转变为无色查尔酮形式在530 nm处无吸收峰,利用此特性分别测定在不同pH条件下530 nm和700 nm处吸光值,即可定量检测植物花色苷的含量。
  • 检测方法: pH示差法
  • 检测波长: 530 nm & 700 nm
  • 信号响应: 递增型
Notices

①A1应控制在0.1-1(96孔板0.05-0.5)范围内,可提高检测灵敏度:若A1大于1(96孔板大于0.5),建议适当减少反应体系中待测样本的添加量,保持总体积200 μL不变(如10 μL待测样本和190 μL试剂一/试剂二);若A1小于0.1(96孔板小于0.05),建议适当增加反应体系中待测样本的添加量,保持总体积200 μL不变(如100 μL待测样本和100 μL试剂一/试剂二),计算时相应修改V样的体积;

②提取液会使蛋白变性,若使用蛋白浓度计算需用PBS单独提取后进行测定;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Hao Y, Wang Q, Abbas M, et al. PagMYB128 regulates anthocyanin biosynthesis by directly activating PagMYB116 in Populus alba× Populus glandulosa[J]. Industrial Crops and Products, 2024, 218: 119046. (IF 5.6)

[2] Zeng Z, Zou Y, Lin F C, et al. Roles of CcDFR and CcOMT9 in the cyanidin biosynthesis and development of Cordyceps cicadae[J]. Frontiers in Microbiology, 2024, 15: 1353710. (IF 5.2)

[3] Cheng H, Chang S, Shi X, et al. Molecular Mechanisms of the Effects of Sodium Selenite on the Growth, Nutritional Quality, and Species of Organic Selenium in Dandelions[J]. Horticulturae, 2024, 10(3): 209. (IF 3.1)

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Concentration (start)xVolume (start)= Concentration (final)× Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
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