Plant Flavonoids Content Assay Kit
植物类黄酮含量检测试剂盒
货号:AKPL015M
规格: 120T/50S
检测设备:酶标仪
可检测样本数:50 Samples
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Product Information
Plant Flavonoids Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:酶标仪/96 孔板
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
类黄酮与铝离子能够在碱性亚硝酸盐溶液中形成红色络合物,产物在510 nm处具有特征吸收峰,通过吸光值的变化即可定量检测植物类黄酮的含量。
  • 检测方法: 铝离子比色法
  • 检测波长: 510 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Rutin
  • 参考标准: y=1.2369x-0.0112 (R²=0.9998)
  • 标准线性范围: 0.05-1.0 mg/mL
  • 检测限: 0.01 mg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①若测定吸光值超出标准吸光值线性范围:高于最高值建议将待测样本适当稀释后再进行测定,低于最低值建议适当增加样本量后再进行测定,计算时相应修改;

②提取液中含有蛋白沉淀组分,样本蛋白浓度测定需使用PBS单独提取后再进行测定;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1]Tissue-specific expression of GhnsLTPs identified via GWAS sophisticatedly coordinates disease- and insect-resistance by regulating metabolic flux redirection in cotton[J]. The Plant Journal, 2021.(IF 6.4)

[2] Zhao Y, Yu S, Zhang S, Li Y, Tu Y, Liu M, Jiang L. Microbiome-Metabolomics Insights into the Milk of Lactating Dairy Cows to Reveal the Health-Promoting Effects of Dietary Citrus Peel Extracts on the Mammary Metabolism. Foods. 2022; 11(24):4119. (IF=5.561)

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