Plant Flavonoids Content Assay Kit
植物类黄酮含量检测试剂盒
货号:AKPL015M
规格: 120T/50S
检测设备:酶标仪
可检测样本数:50 Samples
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Product Information
Plant Flavonoids Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:酶标仪/96 孔板
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
类黄酮与铝离子能够在碱性亚硝酸盐溶液中形成红色络合物,产物在510 nm处具有特征吸收峰,通过吸光值的变化即可定量检测植物类黄酮的含量。
  • 检测方法: 铝离子比色法
  • 检测波长: 510 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Rutin
  • 参考标准: y=1.2369x-0.0112 (R²=0.9998)
  • 标准线性范围: 0.05-1.0 mg/mL
  • 检测限: 0.01 mg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①若测定吸光值超出标准吸光值线性范围:高于最高值建议将待测样本适当稀释后再进行测定,低于最低值建议适当增加样本量后再进行测定,计算时相应修改;

②提取液中含有蛋白沉淀组分,样本蛋白浓度测定需使用PBS单独提取后再进行测定;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Chen B, Zhang Y, Sun Z, et al. Tissue‐specific expression of GhnsLTPs identified via GWAS sophisticatedly coordinates disease and insect resistance by regulating metabolic flux redirection in cotton[J]. The Plant Journal, 2021, 107(3): 831-846.(IF 6.486)

[2] Zhao Y, Yu S, Zhang S, et al. Microbiome-Metabolomics Insights into the Milk of Lactating Dairy Cows to Reveal the Health-Promoting Effects of Dietary Citrus Peel Extracts on the Mammary Metabolism[J]. Foods, 2022, 11(24): 4119.(IF 5.561)

[3] Li L, Wu S, Wang S, et al. Molecular Mechanism of Exogenous Selenium Affecting the Nutritional Quality, Species and Content of Organic Selenium in Mustard[J]. Agronomy, 2023, 13(5): 1425.(IF 3.7)

[4] Pu Y T, Wang C, Jiang Y W, et al. Metabolic profiling and transcriptome analysis provide insights into the accumulation of flavonoids in chayote fruit during storage[J]. Frontiers in Nutrition, 2023, 10: 1029745.(IF 5)

[5] Zhao Y, Zhou G, Sun T, et al. Metabolites and Plant Hormones Related to the Resistance Response to Feeding Stimulation and Leaf Clipping Control in Chinese Pine (Pinus tabuliformis Carr.)[J]. Current Issues in Molecular Biology, 2023, 45(2): 1086-1099.(IF 3.1)

[6] Li X, Xiu D, Huang J, et al. Nutshell Physicochemical Characteristics of Different Hazel Cultivars and Their Defensive Activity toward Curculio nucum (Coleoptera: Curculionidae)[J]. Forests, 2023, 14(2): 319.(IF 2.9)

[7] Chen Z, Wang A, Qin W, et al. Study on the microbial inactivation of whole hulless barley flour using a continuous instant pressure drop sterilizer[J]. Journal of Food Process Engineering, 2022, 45(12): e14169.(IF 2.889)

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