Soil Sucrase (S-SC) Activity Assay Kit
土壤蔗糖酶活性检测试剂盒
货号:AKEN019M
规格: 120T/50S
检测设备:酶标仪
可检测样本数:50 Samples
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Product Information
Soil Sucrase (S-SC) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:酶标仪/96 孔板
  • 预计测定时间:25 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    甲苯(C7H8,MW=96.14,CAS:108-88-3)
Detection Principle
土壤蔗糖酶催化蔗糖降解产生还原糖,进一步与3,5-二硝基水杨酸反应,生成棕红色氨基化合物,产物在540 nm处具有特征吸收峰,通过吸光值变化即可表征土壤蔗糖酶的活性。
  • 检测方法: DNS比色法
  • 检测波长: 540 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Glucose
  • 参考标准: y=2.4473x-0.1017 (R2=0.9998)
  • 标准线性范围: 0.05-0.7 mg/mL
  • 检测限: To Be Updated
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
①若测定吸光值超出标准线性吸光值范围:高于最高值建议适当扩大上清液稀释倍数后再进行后续测定,低于最低值建议减小上清液稀释倍数或适当增加样本量后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Xiao J, Lan S, Zhang Z, et al. Physical disturbance reduces cyanobacterial relative abundance and substrate metabolism potential of biological soil crusts on a gold mine tailing of central China[J]. Frontiers in Microbiology, 2022, 13: 811039.(IF 6.064)

[2] Du Y, Zhang Q, Yu M, et al. Effect of sodium alginate–gelatin–polyvinyl pyrrolidone microspheres on cucumber plants, soil, and microbial communities under lead stress[J]. International Journal of Biological Macromolecules, 2023, 247: 125688.(IF 8.2)

[3] Xiao J, Lan S, Farías M E, et al. The living forms of Microcoleus vaginatus and their contributions to the aggregate structure of biocrusts[J]. FEMS Microbiology Ecology, 2023, 99(5): fiad040.(IF 4.2)

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