Reactive Oxygen Species (ROS)Assay Kit
活性氧检测试剂盒
货号:AKCE002-1
规格: 100-500T(绿色荧光)
检测设备:荧光分光光度计
可检测样本数:100-500 Samples
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Product Information
Reactive Oxygen Species (ROS)Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:100-500 Samples
  • 主要检测设备及配套:荧光显微镜/荧光分光光度计/酶标仪或流式细胞仪检测
  • 预计测定时间:-
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
利用荧光探针DCFH-DA进行活性氧检测是一种基于荧光强度变化,定量检测细胞内活性氧水平的常用方法,DCFH-DA本身没有荧光,可以自由穿过细胞膜,进入细胞内后,被细胞内的酯酶水解生成DCFH,而DCFH不能通透细胞膜,从而使探针易于装载至细胞内,细胞内的活性氧可以氧化无荧光的DCFH生成有荧光的DCF,在最大激发波长480nm,最大发射波长525nm处,使用荧光显微镜,流式细胞仪或激光共聚焦显微镜等检测荧光信号,并根据阳性对照Rosup(浓度为50 mg/mL)的荧光信号来分析活性氧的真正水平。
  • 检测方法: DCFH-DA探针法
  • 检测波长: 488 nm & 525 nm
  • 信号响应: 递增型
Notices

①探针装载后,一定要洗净残余的未进入细胞内的探针,否则会导致背景较高;

②探针装载完毕并洗净残余探针后,可以进行激发波长的扫描和发射波长的扫描,以确认探针的装载情况是否良好;

③尽量缩短探针装载后到测定所用的时间(刺激时间除外),以减少各种可能的误差;

④为了您的安全和健康,请穿实验服并戴一次性手套操作;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Huang Y, Kou Q, Su Y, et al. Combination therapy based on dual-target biomimetic nano-delivery system for overcoming cisplatin resistance in hepatocellular carcinoma[J]. Journal of Nanobiotechnology, 2023, 21(1): 89.(IF 10.2)

[2] Xie J, Hu Y, Su W, et al. PLGA nanoparticles engineering extracellular vesicles from human umbilical cord mesenchymal stem cells ameliorates polyethylene particles induced periprosthetic osteolysis[J]. Journal of Nanobiotechnology, 2023, 21(1): 398.(IF 10.2)

[3] Ji C, Miao J, Xia B, et al. Evaluation of the toxic effects of fluindapyr, a novel SDHI fungicide, to the earthworms Eisenia fetida[J]. Science of The Total Environment, 2023, 899: 165697.(IF 9.8)

[4] Xie J, Hu Y, Li H, et al. Targeted therapy for peri-prosthetic osteolysis using macrophage membrane-encapsulated human urine-derived stem cell extracellular vesicles[J]. Acta Biomaterialia, 2023, 160: 297-310.(IF 9.7)

[5] Zhu Y, Lin K, Zhang X, et al. Antibacterial Effect of Gallic Acid in UV-C Light Treatment Against Escherichia coli O157: H7 and the Underlying Mechanism[J]. Food and Bioprocess Technology, 2023: 1-16.(IF 5.6)

[6] Du P, Li N, Wang J, et al. Structural Characterization and Antifatigue Activity of a Novel Exopolysaccharide Isolated from Marasmius androsaceus[J]. Journal of Food Biochemistry, 2023, 2023.(IF 4)

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