Starch Phosphorylase (SP) Activity Assay Kit
淀粉磷酸化酶活性检测试剂盒
货号:AKSU082U
规格: 50T/48S
检测设备:紫外分光光度计
可检测样本数:48 Samples
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Product Information
Starch Phosphorylase (SP) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:48 Samples
  • 主要检测设备及配套:紫外分光光度计/1 mL石英比色皿(d=10 mm)
  • 预计测定时间:4 h (48 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
淀粉磷酸化酶可催化淀粉中的α-1,4-糖苷键与无机磷反应产生葡萄糖-1-磷酸,葡萄糖-1-磷酸在磷酸葡萄糖变位酶的作用下产生葡萄糖-6-磷酸,并在6-磷酸葡萄糖脱氢酶催化下还原NADP+产生NADPH,NADPH在340 nm处具有特征吸收峰,通过吸光值变化即可表征淀粉磷酸化酶的活性。
  • 检测方法: NADPH速率法
  • 检测波长: 340 nm
  • 信号响应: 递增型
Notices
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
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