

①待测样本需置于冰上放置,且柠檬酸提取过程需在冰上进行;
②若测定吸光值超出标准线性吸光值范围:高于最高值建议将待测样本适当稀释后再进行测定;低于最低值建议适当增加样本量重新提取后再进行测定,计算时相应修改;
③试剂五为易致癌物质,实验过程中需佩戴手套并做好防护,避免皮肤接触;
④柠檬酸提取液不能用于蛋白含量测定,如需测定蛋白含量,应另取组织进行测定;
⑤若显色30 min后出现明显黑色颗粒,属于正常现象,需将待测样本适当稀释后重新进行测定;
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