L-Lactic Acid (L-LA) Content Assay Kit
L-乳酸含量检测试剂盒
货号:AKAC001C
规格: 70T/25S
检测设备:可见分光光度计
可检测样本数:25 Samples
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Product Information
L-Lactic Acid (L-LA) Content Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:25 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:4 h (25 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    无水乙醇(C2H6O,MW=46.07,CAS: 64-17-5)
Detection Principle
L-乳酸在L-乳酸脱氢酶的作用下生成丙酮酸,同时使NAD+还原生成NADH和H+,PMS递H+使MTT还原为紫色物质,产物在570 nm处具有特征吸收峰,通过吸光值变化即可定量检测乳酸的含量。
  • 检测方法: MTT 还原法
  • 检测波长: 570 nm
  • 信号响应: 递增型
Notices
①若测定吸光值超出标准线性吸光值范围,可以增加样本量或适当稀释样本后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] He Y, Jiang S, Zhong Y, et al. USP7 promotes non‐small‐cell lung cancer cell glycolysis and survival by stabilizing and activating c‐Abl[J]. Clinical and Translational Medicine, 2023, 13(12): e1509.(IF 10.6)

[2] Liu W X, Wang J J, Xiao X K, et al. Antimicrobial effects and metabolomics analysis of cell-free supernatant produced by Pediococcus acidilactici LWX 401 isolated from Yunnan traditional pickles[J]. LWT, 2024, 191: 115626.(IF 6)

[3] Zhou T, Xiao Z, Lu J, et al. IGF2BP3-mediated regulation of GLS and GLUD1 gene expression promotes treg-induced immune escape in human cervical cancer[J]. American Journal of Cancer Research, 2023, 13(11): 5289.(IF 5.3)

[4] Yu J, Jiang W, Wang S, et al. Microencapsulated hawthorn berry polyphenols alleviate exercise fatigue in mice by regulating AMPK signaling pathway and balancing intestinal microflora[J]. Journal of Functional Foods, 2022, 97: 105255.(IF 5.223)

[5] Du P, Li N, Wang J, et al. Structural Characterization and Antifatigue Activity of a Novel Exopolysaccharide Isolated from Marasmius androsaceus[J]. Journal of Food Biochemistry, 2023, 2023.(IF 4.0)

[6] Li P, Tang C, Fu D, et al. Cardamonin suppresses glycolysis and induces oxidative stress by inhibiting PI3K/AKT/mTOR pathway in bladder cancer cells[J]. Tropical Journal of Pharmaceutical Research, 2023, 22(8): 1541-1546.(IF 0.6)

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Concentration (start)xVolume (start)= Concentration (final)× Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
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