L-Lactic Acid (L-LA) Content Assay Kit (MTT Method)
L-乳酸含量检测试剂盒（MTT法）
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Product Information
L-Lactic Acid (L-LA) Content Assay Kit (MTT Method)
-20℃ Dry Ice Transportation
- 检测样本量：50 Samples
- 主要检测设备及配套：酶标仪/96孔板
- 预计测定时间：4 h (50 Samples)
- 试剂储存条件：按照标签要求储存
-
需自备试剂:无
Detection Principle
L-乳酸脱氢酶（L-LDH）能够催化L-乳酸生成丙酮酸，同时使NAD+还原生成NADH和H+，进一步通过PMS的递氢作用，还原氧化型噻唑蓝（MTT）生成甲臜，产物在570 nm处具有特征吸收峰，通过吸光值变化即可定量检测L-乳酸的含量。
- 检测方法： MTT还原法
-
检测波长： 570 nm
-
信号响应： 递增型
Refreence Information
- 标准物质： L-Lactic Acid
- 参考标准： y=0.4425x-0.0139 (R2=0.9994)
- 标准线性范围： 0.2-2.5 µmol/mL
- 检测限： 0.2 µmol/mL
- 注：不同仪器及比色材质会对结果产生影响，以实际测定值为准。
Notices
①若A测定或∆A测定超出标准吸光值线性范围：高于最高值建议将待测样本使用蒸馏水适当稀释后再进行测定；低于最低值建议制备更高浓度样本后再进行测定，计算时相应修改；
②试剂三、试剂五和试剂六配制后有效期较短，为便于试验安排，均附赠一瓶作为备用，每瓶均可完成至少50个样本的检测；
③提取液中含有蛋白沉淀组分，待测样本不能用于蛋白含量测定，若使用蛋白浓度计算L-乳酸含量，则需要另取样本使用PBS或生理盐水按照相同步骤制备为待测样本，再进行蛋白浓度测定；
④实验过程中弃上清液时应避免吸入沉淀物而影响结果的准确性；
⑤准确在规定时间点完成吸光值测定，以确保检测结果的准确性和重复性；若样本较多可分批进行检测，以确保组间反应时间一致；
⑥反应体系中试剂不能按比例配制为混合液使用，必须按反应体系顺序依次加入；
注： 为保证结果准确且避免试剂损失，测定前请仔细阅读说明书（以实际收到说明书为准），确认试剂储存和准备是否充分，操作步骤是否清楚，且务必取2-3个预期差异交的样本进行预测定，过程中问题请您及时与工作人员联系。
Product Citation
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[10] Zhao L, Zhang J, He J, et al. Biochemical impact of ALAEm supplementation in late gestation on the reproductive performance of sows[J]. Frontiers in Veterinary Science, 2025, 12: 1548263. (IF 2.6)
[11] Cui H, Ma Y, Han S, et al. Arsenic trioxide regulates the glycolytic pathway to treat acute promyelocytic leukemia by inhibiting RPL22L1[J]. Leukemia Research, 2024: 107550. (IF 2.1)
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The Dilution Calculator Equation
Concentration (start)xVolume (start)= Concentration (final)× Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
This equation is commonly abbreviated as: C1V1 = C2V2
Sample(S):可测定的样品数量；
★根据此数量计算需要的试剂盒个数。
Size
120T/50S
Test(T):总反应次数；
★每个试剂盒均预留了至少5个（分光光度法）和10个（微板法）样本预实验的试剂用量；
★若需要制作标准曲线则至少能够满足2次制作标准曲线的试剂用量（15个）。
